ARG65448

anti-CD4 antibody [GK1.5] (PE)

anti-CD4 antibody [GK1.5] (PE) for Flow cytometry,IHC-Frozen sections,ICC/IF,Depletion,Blocking and Mouse

Developmental Biology antibody; Immune System antibody

Overview

Product Description PE-conjugated Rat Monoclonal antibody [GK1.5] recognizes CD4
Tested Reactivity Ms
Tested Application BL, Depletion, FACS, ICC/IF, IHC-Fr
Host Rat
Clonality Monoclonal
Clone GK1.5
Isotype IgG2bκ
Target Name CD4
Antigen Species Mouse
Immunogen Mouse CTL clone V4
Conjugation PE
Full Name CD4 molecule
Alternate Names CD4mut; CD antigen CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

Application Instructions

Application Suggestion
Application Dilution
BLAssay-dependent
DepletionAssay-dependent
FACS< 0.2 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS, Sodium azide and Sucrose
Preservative Sodium azide
Stabilizer Sucrose
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12504 Mouse CD4

Swiss-port # P06332 Mouse T-cell surface glycoprotein CD4

Gene Symbol CD4
Gene Full Name CD4 molecule
Background CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Research Area Developmental Biology antibody; Immune System antibody
PTM Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Images (7) Click the Picture to Zoom In

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21993 anti-CD8a antibody [53-6.7] (Biotin) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21994 anti-CD8a antibody [53-6.7] (Spectral Red) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21996 anti-CD8a antibody [53-6.7] (PE/Cy5.5) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21997 anti-CD8a antibody [53-6.7] (PE-Cy7) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20854 anti-CD19 antibody [6D5] (Spectral Red) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    FACS: BALB/c Mouse splenocytes stained with ARG22352 anti-CD8a antibody [53-6.7] (FITC) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG65448 anti-CD4 antibody [GK1.5] (PE) and ARG62940 anti-CD8a antibody [53-6.7] (FITC).

Clone References

Myxoma virus expressing a fusion protein of interleukin-15 (IL15) and IL15 receptor alpha has enhanced antitumor activity.

IHC-Fr / Mouse

Tosic V et al.
PLoS One.,  (2014)

publication_link

 

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Autologous tumor vaccine modified with recombinant new castle disease virus expressing IL-7 promotes antitumor immune response.

Zhao L et al.
J Immunol.,  (2014)

publication_link

 

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Supplementation of influenza split vaccines with conserved M2 ectodomains overcomes strain specificity and provides long-term cross protection.

Kim MC et al.
Mol Ther.,  (2014)

publication_link

 

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Enhancement of systemic tumor immunity for squamous cell carcinoma cells by an oncolytic herpes simplex virus.

IHC-Fr / Mouse

Meshii N et al.
Cancer Gene Ther.,  (2013)

publication_link

 

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A quantitative study of the mechanisms behind thymic atrophy in Gαi2-deficient mice during colitis development.

FACS / Mouse

Elgbratt K et al.
PLoS One.,  (2012)

publication_link

 

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