ARG65448

anti-CD4 antibody [GK1.5] (PE)

anti-CD4 antibody [GK1.5] (PE) for Flow cytometry,IHC-Frozen sections,ICC/IF,Depletion,Blocking and Mouse

Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

概述

产品描述 PE-conjugated Rat Monoclonal antibody [GK1.5] recognizes CD4
反应物种 Ms
应用 BL, Depletion, FACS, ICC/IF, IHC-Fr
宿主 Rat
克隆 Monoclonal
克隆号 GK1.5
同位型 IgG2b, kappa
靶点名称 CD4
抗原物种 Mouse
抗原 Mouse CTL clone V4
偶联标记 PE
全名 CD4 molecule
別名 CD4mut; CD antigen CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

应用说明

应用建议
应用 推荐稀释比
BLAssay-dependent
DepletionAssay-dependent
FACS< 0.2 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 51 kDa

属性

形式 Liquid
缓冲液 PBS, Sodium azide and Sucrose
抗菌剂 Sodium azide
稳定剂 Sucrose
浓度 0.1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12504 Mouse CD4

Swiss-port # P06332 Mouse T-cell surface glycoprotein CD4

基因名称 CD4
基因全名 CD4 molecule
背景介绍 CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
生物功能 CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. [UniProt]
产品亮点 Related products:
CD4 antibodies; CD4 ELISA Kits; CD4 Duos / Panels; Anti-Rat IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
研究领域 Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
翻译后修饰 Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

检测图片 (7) Click the Picture to Zoom In

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21993 anti-CD8a antibody [53-6.7] (Biotin) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21994 anti-CD8a antibody [53-6.7] (Spectral Red) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21996 anti-CD8a antibody [53-6.7] (PE/Cy5.5) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21997 anti-CD8a antibody [53-6.7] (PE-Cy7) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20854 anti-CD19 antibody [6D5] (Spectral Red) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    FACS: BALB/c Mouse splenocytes stained with ARG22352 anti-CD8a antibody [53-6.7] (FITC) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG65448 anti-CD4 antibody [GK1.5] (PE) and ARG62940 anti-CD8a antibody [53-6.7] (FITC).

克隆号文献

Myxoma virus expressing a fusion protein of interleukin-15 (IL15) and IL15 receptor alpha has enhanced antitumor activity.

IHC-Fr / Mouse

Tosic V et al.
PLoS One.,  (2014)

publication_link

 

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Autologous tumor vaccine modified with recombinant new castle disease virus expressing IL-7 promotes antitumor immune response.

Zhao L et al.
J Immunol.,  (2014)

publication_link

 

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Supplementation of influenza split vaccines with conserved M2 ectodomains overcomes strain specificity and provides long-term cross protection.

Kim MC et al.
Mol Ther.,  (2014)

publication_link

 

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Enhancement of systemic tumor immunity for squamous cell carcinoma cells by an oncolytic herpes simplex virus.

IHC-Fr / Mouse

Meshii N et al.
Cancer Gene Ther.,  (2013)

publication_link

 

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A quantitative study of the mechanisms behind thymic atrophy in Gαi2-deficient mice during colitis development.

FACS / Mouse

Elgbratt K et al.
PLoS One.,  (2012)

publication_link

 

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