ARG65448

anti-CD4 antibody [GK1.5] (PE)

anti-CD4 antibody [GK1.5] (PE) for Flow cytometry,IHC-Frozen sections,ICC/IF,Depletion,Blocking and Mouse

Developmental Biology antibody; Immune System antibody

概述

产品描述 PE-conjugated Rat Monoclonal antibody [GK1.5] recognizes CD4
反应物种 Ms
应用 BL, Depletion, FACS, ICC/IF, IHC-Fr
宿主 Rat
克隆 Monoclonal
克隆号 GK1.5
同位型 IgG2bκ
靶点名称 CD4
抗原物种 Mouse
抗原 Mouse CTL clone V4
偶联标记 PE
全名 CD4 molecule
別名 CD4mut; CD antigen CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

应用说明

应用建议
应用 推荐稀释比
BLAssay-dependent
DepletionAssay-dependent
FACS< 0.2 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 PBS, Sodium azide and Sucrose
抗菌剂 Sodium azide
稳定剂 Sucrose
浓度 0.1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12504 Mouse CD4

Swiss-port # P06332 Mouse T-cell surface glycoprotein CD4

基因名称 CD4
基因全名 CD4 molecule
背景介绍 CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
研究领域 Developmental Biology antibody; Immune System antibody
翻译后修饰 Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

检测图片 (7) Click the Picture to Zoom In

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21993 anti-CD8a antibody [53-6.7] (Biotin) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21994 anti-CD8a antibody [53-6.7] (Spectral Red) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21996 anti-CD8a antibody [53-6.7] (PE/Cy5.5) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21997 anti-CD8a antibody [53-6.7] (PE-Cy7) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20854 anti-CD19 antibody [6D5] (Spectral Red) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    FACS: BALB/c Mouse splenocytes stained with ARG22352 anti-CD8a antibody [53-6.7] (FITC) and ARG65448 anti-CD4 antibody [GK1.5] (PE).

  • ARG65448 anti-CD4 antibody [GK1.5] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG65448 anti-CD4 antibody [GK1.5] (PE) and ARG62940 anti-CD8a antibody [53-6.7] (FITC).

克隆号文献

Myxoma virus expressing a fusion protein of interleukin-15 (IL15) and IL15 receptor alpha has enhanced antitumor activity.

IHC-Fr / Mouse

Tosic V et al.
PLoS One.,  (2014)

publication_link

 

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Autologous tumor vaccine modified with recombinant new castle disease virus expressing IL-7 promotes antitumor immune response.

Zhao L et al.
J Immunol.,  (2014)

publication_link

 

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Supplementation of influenza split vaccines with conserved M2 ectodomains overcomes strain specificity and provides long-term cross protection.

Kim MC et al.
Mol Ther.,  (2014)

publication_link

 

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Enhancement of systemic tumor immunity for squamous cell carcinoma cells by an oncolytic herpes simplex virus.

IHC-Fr / Mouse

Meshii N et al.
Cancer Gene Ther.,  (2013)

publication_link

 

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A quantitative study of the mechanisms behind thymic atrophy in Gαi2-deficient mice during colitis development.

FACS / Mouse

Elgbratt K et al.
PLoS One.,  (2012)

publication_link

 

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