ARG67079

anti-CD3D antibody [SQab30351]

anti-CD3D antibody [SQab30351] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

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概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab30351] recognizes CD3D
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab30351
同位型 IgG
靶点名称 CD3D
抗原物种 Human
抗原 Synthetic peptide of CD3D.
偶联标记 Un-conjugated
別名 CD3D; CD3 Delta Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Delta Chain; CD3-DELTA; CD3DELTA; T3D; CD3d Antigen, Delta Polypeptide (TiT3 Complex); CD3d Molecule, Delta (CD3-TCR Complex); T-Cell Receptor T3 Delta Chain; CD3 Antigen, Delta Subunit; OKT3, Delta Chain; CD3d Molecule; CD3d Antigen; CD3 Delta; IMD19

应用说明

应用建议
应用 推荐稀释比
IHC-P1:100-1:200
WB1:500 - 1:2000
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 20 -25 kDa

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12500 Mouse CD3D

GeneID: 25710 Rat CD3D

GeneID: 915 Human CD3D

基因名称 CD3D
全名 CD3 Delta Subunit Of T-Cell Receptor Complex
背景介绍 The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.
生物功能 Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways.
细胞定位 Cell membrane, Membrane
预测分子量 19 kDa
翻译后修饰 Disulfide bond, Glycoprotein, Phosphoprotein

检测图片 (2) Click the Picture to Zoom In

  • ARG67079 anti-CD3D antibody [SQab30351] IHC-P image

    Immunohistochemistry: Human tonsil stained with ARG67079 anti-CD3D antibody [SQab30351].

  • ARG67079 anti-CD3D antibody [SQab30351] WB image (Customer review)

    Western blot: Jurkat stained with ARG67079 anti-CD3D antibody [SQab30351] at 1:500 dilution.

客户反馈

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anti-CD3D antibody [SQab30351]

Application:WB

Sample:Rat Brain

Sample Loading Amount:10 μL

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-CD3D antibody [SQab30351]

Application:WB

Sample:Mouse Kidney

Sample Loading Amount:10 μL

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-CD3D antibody [SQab30351]

Application:WB

Sample:MOLT4

Sample Loading Amount:10 μL

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC