ARG52756
anti-CD34 antibody [QBEND/10]
anti-CD34 antibody [QBEND/10] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Primates,Rabbit
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
参考文献2
概述
| 产品描述 | Mouse Monoclonal antibody [QBEND/10] recognizes CD34 (Endothelial Cell Marker) |
|---|---|
| 反应物种 | Hu, Ms, NHuPrm, Rb |
| 不反应物种 | Rat, Bov, Dog, Sheep |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 特异性 | The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND/10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. The clone QBEND/10 recognises the Class II epitope on CD34. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | QBEND/10 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD34 |
| 抗原物种 | Human |
| 抗原 | Detergent solubilized vesicular suspension prepared from a perfusate of human term placenta. |
| 偶联标记 | Un-conjugated |
| 別名 | Hematopoietic progenitor cell antigen CD34; CD antigen CD34 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Lung, Tonsil | ||||||||||||||
| 实际分子量 | 105-120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | Tissue culture supernatant in TBS (pH 7.5), 1% BSA and <0.1% Sodium azide |
| 抗菌剂 | <0.1% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34 Swiss-port # Q64314 Mouse Hematopoietic progenitor cell antigen CD34 |
|---|---|
| 基因名称 | CD34 |
| 全名 | CD34 molecule |
| 背景介绍 | CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| 生物功能 | CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt] |
| 细胞定位 | Cytoplasm, Membrane |
| 产品亮点 | Related Antibody Duos and Panels: ARG30300 Endothelial Cell Marker Antibody Duo (CD31, CD34) ARG30328 Angiogenesis Antibody Panel Related products: CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Vascular development is regulated by FGF-dependent metabolic control |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody |
| 预测分子量 | 41 kDa |
| 翻译后修饰 | Highly glycosylated. Phosphorylated on serine residues by PKC. |
检测图片 (8) Click the Picture to Zoom In
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ARG52756 anti-CD34 antibody [QBEND/10] ICC/IF image
Immunofluorescence: hCMEC/D3 cells stained with ARG52756 anti-CD34 antibody [QBEND/10].
From Lucie Y Li et al. Front Cell Neurosci. (2023), doi: 10.3389/fncel.2023.1077204, Fig. 3A & B.
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ARG52756 anti-CD34 antibody [QBEND/10] WB image
Western blot: 30 µg of mouse lung lysate stained with ARG52756 anti-CD34 antibody [QBEND/10] at 1:500 dilution.
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Breast Ductal Carcinoma stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Cervical Squamous Cell carcinoma stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Colon stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Placenta stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human Tonsil stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)
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ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image
Immunohistochemistry: Human colorectal cancer stained with ARG52756 anti-CD34 antibody [QBEND/10].
From Wu P et al. Dig Dis Sci- (2018), doi: 10.1007/s10620-017-4818-1, Fig. 3. A.
客户反馈
Good
anti-CD34 antibody [QBEND/10]
Application：WB
Sample：Mouse lung
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Brain blood vessel autoantibodies in patients with NMDA and GABAA receptor encephalitis: identification of unconventional Myosin-X as target antigen
ICC/IF / Human
Role of Angiogenesis in Chronic Radiation Proctitis: New Evidence Favoring Inhibition of Angiogenesis Ex Vivo.
IHC-P / Human
