ARG65379

anti-CD314 / NKG2D antibody [1D11] (FITC)

Datasheet

订购信息

包装

价格 (USD)

100 tests

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	FITC-conjugated Mouse Monoclonal antibody [1D11] recognizes CD314 / NKG2D
反应物种	Hu
应用	FACS
特异性	The clone 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.
宿主	Mouse
克隆	Monoclonal
克隆号	1D11
同位型	IgG1
靶点名称	CD314 / NKG2D
抗原	NKL cell line
偶联标记	FITC
別名	NKG2-D-activating NK receptor; CD antigen CD314; D6H12S2489E; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D; Killer cell lectin-like receptor subfamily K member 1; Nkg2d

应用说明

应用建议

应用	推荐稀释比
FACS	4 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 22914 Human KLRK1 Swiss-port # P26718 Human NKG2-D type II integral membrane protein
基因名称	KLRK1
全名	killer cell lectin-like receptor subfamily K, member 1
背景介绍	CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.
生物功能	Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. [UniProt]
研究领域	Immune System antibody
预测分子量	25 kDa

检测图片 (3) Click the Picture to Zoom In

ARG65379 anti-CD314 / NKG2D antibody [1D11] (FITC) FACS image

Flow Cytometry: Human peripheral whole blood stained with ARG65379 anti-CD314 / NKG2D antibody [1D11] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).
ARG65379 anti-CD314 / NKG2D antibody [1D11] (FITC) FACS image

Flow Cytometry: Human lymphocytes stained with ARG65379 anti-CD314 / NKG2D antibody [1D11] (FITC) (4 µl reagent / 100 µl of peripheral whole blood) and anti-CD56 antibody [LT56] (APC) (10 µl reagent / 100 µl of peripheral whole blood).
ARG65379 anti-CD314 / NKG2D antibody [1D11] (FITC) FACS image

Flow Cytometry: Separation of human CD314 / NKG2D positive CD56 positive NK cells (red-filled) from CD314 / NKG2D negative CD56 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65379 anti-CD314 / NKG2D antibody [1D11] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).