ARG65378
anti-CD314 / NKG2D antibody [1D11]
anti-CD314 / NKG2D antibody [1D11] for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation and Human
Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [1D11] recognizes CD314 / NKG2D |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IHC-Fr, IP |
| 特异性 | The clone 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1D11 |
| 同位型 | IgG1 |
| 靶点名称 | CD314 / NKG2D |
| 抗原 | NKL cell line |
| 偶联标记 | Un-conjugated |
| 別名 | NKG2-D-activating NK receptor; CD antigen CD314; D6H12S2489E; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D; Killer cell lectin-like receptor subfamily K member 1; Nkg2d |
应用说明
| 应用建议 |
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| 应用说明 | Functional studies: Blocking of ligand binding. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P26718 Human NKG2-D type II integral membrane protein |
|---|---|
| 基因名称 | KLRK1 |
| 全名 | killer cell lectin-like receptor subfamily K, member 1 |
| 背景介绍 | CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified. |
| 生物功能 | Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. [UniProt] |
| 研究领域 | Immune System antibody |
| 预测分子量 | 25 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG65378 anti-CD314 / NKG2D antibody [1D11] FACS image
Flow Cytometry: Human peripheral blood stained with ARG65378 anti-CD314 / NKG2D antibody [1D11] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65378 anti-CD314 / NKG2D antibody [1D11] FACS image
Flow Cytometry: Separation of human CD314 / NKG2D positive lymphocytes (red-filled) from CD314 / NKG2D negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65378 anti-CD314 / NKG2D antibody [1D11] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
In vitro Natural Killer Cell Immunotherapy for Medulloblastoma.
FACS / Human
Rescue of impaired NK cell activity in hodgkin lymphoma with bispecific antibodies in vitro and in patients.
FACS / Human
Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells.
FuncSt / Human
Genome-wide association study in alopecia areata implicates both innate and adaptive immunity.
IHC-Fr / Human
