ARG66342

anti-CD31 antibody [SQab18116]

Datasheet

参考文献2

订购信息

包装

价格 (USD)

100 μl

399
258

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Recombinant Rabbit Monoclonal antibody [SQab18116] recognizes CD31
反应物种	Hu
应用	FACS, IHC-P, IP, WB
宿主	Rabbit
克隆	Monoclonal
克隆号	SQab18116
同位型	IgG
靶点名称	CD31
抗原物种	Human
抗原	Synthetic peptide corresponding to residues on the C-terminus of Human CD31.
偶联标记	Un-conjugated
別名	EndoCAM; CD31/EndoCAM; PECAM-1; CD31; PECA1; CD antigen CD31; GPIIA'; endoCAM; Platelet endothelial cell adhesion molecule

应用说明

应用建议

应用	推荐稀释比
FACS	1:200 - 1:1000
IHC-P	1:1600 - 1:3200
IP	1:50
WB	1:1000 - 1:2000

应用说明

IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

实际分子量

~ 140 kDa

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂	0.01% Sodium azide
稳定剂	40% Glycerol and 0.05% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 5175 Human PECAM1 Swiss-port # P16284 Human Platelet endothelial cell adhesion molecule
基因名称	PECAM1
全名	platelet/endothelial cell adhesion molecule 1
背景介绍	CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
生物功能	CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. [UniProt]
产品亮点	Related products: CD31 antibodies; CD31 ELISA Kits; CD31 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones)
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody
预测分子量	83 kDa
翻译后修饰	Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. [UniProt]

检测图片 (5) Click the Picture to Zoom In

ARG66342 anti-CD31 antibody [SQab18116] FACS image

Flow Cytometry: Jurkat cells fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG66342 anti-CD31 antibody [SQab18116] (red) at 1:1000 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
ARG66342 anti-CD31 antibody [SQab18116] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human placenta stained with ARG66342 anti-CD31 antibody [SQab18116] at 1:3200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
ARG66342 anti-CD31 antibody [SQab18116] WB image

Western blot: 10 µg of A431 cell lysate stained with ARG66342 anti-CD31 antibody [SQab18116] at 1:1000 dilution.
ARG66342 anti-CD31 antibody [SQab18116] IP image

Immunoprecipitation: 0.4 mg of THP-1 whole cell lysate was immunoprecipitated (1:50 dilution) and stained with ARG66342 anti-CD31 antibody [SQab18116].

Lane 1: Immunoprecipitation in THP-1 whole cell lysate
Lane 2: PBS instead of Primary Ab in THP-1 whole cell lysate
Lane 3: THP-1 whole cell lysate, 10 µg (input)
ARG66342 anti-CD31 antibody [SQab18116] WB image

Western blot: 10 µg of THP-1 cell lysate stained with ARG66342 anti-CD31 antibody [SQab18116] at 1:1000 dilution.