ARG56119
anti-CD31 antibody [JC/70A]
anti-CD31 antibody [JC/70A] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody [JC/70A] recognizes CD31 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rb |
| 不反应物种 | Rat, Pig |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | JC/70A |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD31 |
| 抗原物种 | Human |
| 抗原 | A membrane preparation of a spleen from a patient with hairy cell leukemia. |
| 偶联标记 | Un-conjugated |
| 別名 | EndoCAM; CD31/EndoCAM; PECAM-1; CD31; PECA1; CD antigen CD31; GPIIA'; endoCAM; Platelet endothelial cell adhesion molecule |
应用说明
| 预测反应性说明 | Duo to the publication PMID: 35242369, this antibody might react to rabbit CD31. | ||||||||||
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| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | HUVEC | ||||||||||
| 实际分子量 | 110 - 120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P16284 Human Platelet endothelial cell adhesion molecule Swiss-port # Q08481 Mouse Platelet endothelial cell adhesion molecule |
|---|---|
| 基因名称 | PECAM1 |
| 全名 | platelet/endothelial cell adhesion molecule 1 |
| 背景介绍 | CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010] |
| 生物功能 | CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. [UniProt] |
| 细胞定位 | Cell surface and cytoplasm of endothelial cells |
| 产品亮点 | Related products: CD31 antibodies; CD31 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Besides tumor suppression, what’s p53 busy for during embryogenesis? |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody |
| 预测分子量 | 83 kDa |
| 翻译后修饰 | Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. |
检测图片 (4) Click the Picture to Zoom In
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ARG56119 anti-CD31 antibody [JC/70A] WB image
Western blot: 10 µg of HUVEC cell lysate stained with ARG56119 anti-CD31 antibody [JC/70A] at 1:200 dilution.
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ARG56119 anti-CD31 antibody [JC/70A] FACS image
Flow Cytometry: Jurkat cells stained with AF488-conjugated ARG56119 anti-CD31 antibody [JC/70A] (green); Cells alone (black); Isotype control (grey).
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ARG56119 anti-CD31 antibody [JC/70A] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human colon carcinoma stained with ARG56119 anti-CD31 antibody [JC/70A].
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ARG56119 anti-CD31 antibody [JC/70A] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human angiosarcoma stained with ARG56119 anti-CD31 antibody [JC/70A].
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anti-CD31 antibody [JC/70A]
Application：WB
Sample：HUVEC
Sample Loading Amount：10 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
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IHC-P / Rabbit
