ARG45111
anti-CD31 antibody
anti-CD31 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CD31 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD31 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human CD31(aa. 218-231). |
| 偶联标记 | Un-conjugated |
| 別名 | PECAM1; Platelet And Endothelial Cell Adhesion Molecule 1; CD31 Antigen; CD31; Platelet Endothelial Cell Adhesion Molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; Platelet/Endothelial Cell Adhesion Molecule 1; Platelet Endothelial Cell Adhesion Molecule-1; CD31/EndoCAM |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Tonsil |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P16284 Human Platelet endothelial cell adhesion molecule |
|---|---|
| 基因名称 | PECAM1 |
| 全名 | Platelet And Endothelial Cell Adhesion Molecule 1 |
| 背景介绍 | CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010] |
| 生物功能 | CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. [UniProt] |
| 细胞定位 | Membrane |
| 产品亮点 | Related Antibody Duos and Panels: ARG30300 Endothelial Cell Marker Antibody Duo (CD31, CD34) ARG30327 Microvascular Density Antibody Panel Related products: CD31 antibodies; CD31 ELISA Kits; CD31 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Microglial help TAM-ing inflammation in the brain SM5-1, a promising immunotherapy for Hepatocellular Carcinoma (HCC) Besides tumor suppression, what’s p53 busy for during embryogenesis? Vascular development is regulated by FGF-dependent metabolic control |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody |
| 预测分子量 | 83 kDa |
| 翻译后修饰 | Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. |
检测图片 (1) Click the Picture to Zoom In
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ARG45111 anti-CD31 antibody ICC/IF image
Immunofluorescence: Jurkat stained with ARG45111 anti-CD31 antibody at 10 µg/ml dilution.
