ARG23469
anti-CD300a + CD300c antibody [P192]
anti-CD300a + CD300c antibody [P192] for Flow cytometry and Human
概述
| 产品描述 | Mouse Monoclonal antibody [P192] recognizes CD300a + CD300c Mouse anti Human CD300a antibody, clone P192 specifically recognizes human CD300a, otherwise known as IRp60 (inhibitory receptor protein 60), a 60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells.Studies have shown that the cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils has been shown to significantly inhibit their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro. |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | P192 |
| 同位型 | IgG2a |
| 靶点名称 | CD300a + CD300c |
| 抗原物种 | Human |
| 抗原 | Polyclonal population of NK cells |
| 偶联标记 | Un-conjugated |
| 別名 | CMRF-35-H9; Inhibitory receptor protein 60; NK inhibitory receptor; CMRF35-H; CMRF35H9; CD antigen CD300a; IRC1/IRC2; IGSF12; CMRF35H; IRp60; IgSF12; CMRF35-like molecule 8; CMRF35-H9; CMRF-35H; CD300 antigen-like family member A; CLM-8; IRC1; IRC2; Immunoglobulin superfamily member 12 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD300A |
| 全名 | CD300a molecule |
| 背景介绍 | This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| 生物功能 | Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. [UniProt] |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine. N-glycosylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG23469 anti-CD300a + CD300c antibody [P192] FACS image
Flow Cytometry: Human peripheral blood monocytes stained with ARG23469 anti-CD300a + CD300c antibody [P192] followed by Rabbit F(ab')2 anti Mouse IgG (FITC).
