ARG53824
anti-CD30 antibody [MEM-268] (APC)
anti-CD30 antibody [MEM-268] (APC) for Flow cytometry and Human
Cancer antibody; Immune System antibody
概述
| 产品描述 | APC-conjugated Mouse Monoclonal antibody [MEM-268] recognizes CD30 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor). |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-268 |
| 同位型 | IgG |
| 靶点名称 | CD30 |
| 抗原 | Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line) |
| 偶联标记 | APC |
| 別名 | Tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30; Ki-1; Lymphocyte activation antigen CD30; CD antigen CD30; D1S166E; CD30L receptor |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P28908 Human Tumor necrosis factor receptor superfamily member 8 |
|---|---|
| 基因名称 | TNFRSF8 |
| 全名 | tumor necrosis factor receptor superfamily, member 8 |
| 背景介绍 | CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| 生物功能 | CD30 is a receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898). [UniProt] |
| 研究领域 | Cancer antibody; Immune System antibody |
| 预测分子量 | 64 kDa |
| 翻译后修饰 | Phosphorylated on serine and tyrosine residues. |
克隆号文献
Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing.
