ARG65442
anti-CD3 zeta phospho (Tyr153) antibody [EM-17]
anti-CD3 zeta phospho (Tyr153) antibody [EM-17] for Flow cytometry,Western blot and Human,Mouse
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [EM-17] recognizes CD3 zeta phospho (Tyr153) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, WB |
| 特异性 | The clone EM-17 recognizes phosphorylated tyrosine 153 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | EM-17 |
| 同位型 | IgG1 |
| 靶点名称 | CD3 zeta |
| 抗原物种 | Mouse |
| 抗原 | A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | IMD25; CD3Z; CD3-ZETA; T-cell surface glycoprotein CD3 zeta chain; CD3Q; T-cell receptor T3 zeta chain; CD3H; TCRZ; T3Z; CD antigen CD247 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Non-reducing condition recommended. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | FACS: Jurkat + pervanadate. WB: Jurkat + pervanadate; Splenocyte of Balb/c or F1 mouse + pervanadate. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P20963 Human T-cell surface glycoprotein CD3 zeta chain Swiss-port # P24161 Mouse T-cell surface glycoprotein CD3 zeta chain |
|---|---|
| 基因名称 | Cd247 |
| 全名 | CD247 antigen |
| 背景介绍 | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
| 生物功能 | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. [UniProt] |
| 产品亮点 | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Phosphorylated on Tyr residues after T-cell receptor triggering. |
检测图片 (1) Click the Picture to Zoom In
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ARG65442 anti-CD3 zeta phospho (Tyr153) antibody [EM-17] WB image
Western blot: Cell lysates with various phosphorylated particular Human CD3 zeta mutants stained with ARG65442 anti-CD3 zeta phospho (Tyr153) antibody [EM-17].
Y1F mutatants lack phosphotyrosine 72, and Y6F mutatants lack phosphotyrosine 153.
