ARG65722
anti-CD3 zeta phospho (Tyr142) antibody [EM-54]
anti-CD3 zeta phospho (Tyr142) antibody [EM-54] for Flow cytometry,Western blot and Human,Mouse
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [EM-54] recognizes CD3 zeta phospho (Tyr142) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, WB |
| 特异性 | This antibody recognizes phosphorylated tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | EM-54 |
| 同位型 | IgG1 |
| 靶点名称 | CD3 zeta |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Tyr142 of Human CD3 zeta. |
| 偶联标记 | Un-conjugated |
| 別名 | IMD25; CD3Z; CD3-ZETA; T-cell surface glycoprotein CD3 zeta chain; CD3Q; T-cell receptor T3 zeta chain; CD3H; TCRZ; T3Z; CD antigen CD247 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P20963 Human T-cell surface glycoprotein CD3 zeta chain Swiss-port # P24161 Mouse T-cell surface glycoprotein CD3 zeta chain |
|---|---|
| 基因名称 | CD247 |
| 全名 | CD247 molecule |
| 背景介绍 | The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. [UniProt] |
| 产品亮点 | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Phosphorylated on Tyr residues after T-cell receptor triggering. |
检测图片 (1) Click the Picture to Zoom In
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ARG65722 anti-CD3 zeta phospho (Tyr142) antibody [EM-54] WB image
Westren blot: Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were stained with ARG65722 anti-CD3 zeta phospho (Tyr142) antibody [EM-54].
Lanes:
1) Wt mCD3zeta pervanadate non-stimulated
2) Wt mCD3zeta pervanadate stimulated
3) mut. mCD3zeta Y/F2-6
4) mut. mCD3zeta Y/F1 and 3-6
5) mut. mCD3zeta Y/F1-2 and 4-6
6) mut. mCD3zeta Y/F1-3 and 5-6
7) mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native)
8) mut. mCD3zeta Y/F1-5.
