ARG55533
anti-CD3 zeta antibody
anti-CD3 zeta antibody for Flow cytometry,Western blot and Human
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD3 zeta |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Pig |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD3 zeta |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 2-31 (N-terminus) of Human CD3 zeta. |
| 偶联标记 | Un-conjugated |
| 別名 | IMD25; CD3Z; CD3-ZETA; T-cell surface glycoprotein CD3 zeta chain; CD3Q; T-cell receptor T3 zeta chain; CD3H; TCRZ; T3Z; CD antigen CD247 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | MOLT-4 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P20963 Human T-cell surface glycoprotein CD3 zeta chain |
|---|---|
| 基因名称 | CD247 |
| 全名 | CD247 molecule |
| 背景介绍 | The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein |
| 产品亮点 | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Phosphorylated on Tyr residues after T-cell receptor triggering. |
检测图片 (2) Click the Picture to Zoom In
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ARG55533 anti-CD3 zeta antibody WB image
Western blot: 20 µg of MOLT-4 whole cell lysate stained with ARG55533 anti-CD3 zeta antibody at 1:1000 dilution.
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ARG55533 anti-CD3 zeta antibody FACS image
Flow Cytometry: Ramos cells stained with ARG55533 anti-CD3 zeta antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
