ARG22832

anti-CD3 epsilon antibody [CA17.2A12] (FITC)

Datasheet

订购信息

包装

价格 (USD)

50 μg

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书

概述

产品描述	FITC-conjugated Mouse Monoclonal antibody [CA17.2A12] recognizes CD3 epsilon CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen expressed by thymocytes and mature T lymphocytes. Clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin.
反应物种	Dog
应用	FACS
宿主	Mouse
克隆	Monoclonal
克隆号	CA17.2A12
同位型	IgG1
靶点名称	CD3 epsilon
抗原物种	Dog
抗原	Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90
偶联标记	FITC
別名	CD3E; CD3 Epsilon Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Epsilon Chain; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); CD3-Epsilon; CD3epsilon; T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3; CD3e Molecule; CD3e Antigen; CD3-EPSILON; CD3EPSILON

应用说明

应用建议

应用	推荐稀释比
FACS	Neat - 1:10

应用说明

FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS, 0.09% Sodium azide and 1% BSA
抗菌剂	0.09% Sodium azide
稳定剂	1% BSA
浓度	0.1 mg/ml
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	CD3E
全名	CD3 Epsilon Subunit Of T-Cell Receptor Complex
背景介绍	The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
生物功能	Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathway.
细胞定位	Cell membrane, Membrane
产品亮点	Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs)
研究领域	Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
预测分子量	23 kDa
翻译后修饰	Disulfide bond, Phosphoprotein

检测图片 (6) Click the Picture to Zoom In

ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) FACS image

Flow Cytometry: Figure A. ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) and RPE conjugated Rat IgG2a isotype control. Figure B. ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) and RPE conjugated Rat anti Canine CD4. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) FACS image

Flow Cytometry: Figure A. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse IgG1 isotype control. Figure B. RPE conjugated Rat anti Canine CD4 and ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) FACS image

Flow Cytometry: Figure A. RPE conjugated Rat anti Canine CD8 and FITC conjugated Mouse IgG1 isotype control. Figure B. RPE conjugated Rat anti Canine CD8 and ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) FACS image

Flow Cytometry: Figure A. ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) and RPE conjugated Rat IgG1 isotype control. Figure B. ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) and RPE conjugated Rat anti Canine CD8. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) FACS image

Flow Cytometry: Figure A. ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) and purified Rat IgG2a isotype control detected with Goat anti Rat IgG2a DyLight 649. Figure B. ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) and purified Mouse anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC) FACS image

Flow Cytometry: Figure A. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649 and Mouse IgG1 FITC isotype control. Figure B. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a (PE) and ARG22832 anti-CD3 epsilon antibody [CA17.2A12] (FITC). All experiments performed on red cell lysed canine blood gated on mononuclear cells.