anti-CD279 / PD-1 antibody [SQab1732]
anti-CD279 / PD-1 antibody [SQab1732] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human
|产品描述||Recombinant Rabbit Monoclonal antibody [SQab1732] recognizes CD279 / PD-1|
|应用||FACS, ICC/IF, IHC-P, IP|
|靶点名称||CD279 / PD-1|
|抗原||Recombinant full length protein of Human PD‐1. The immunogen contains the specific extracellular domain of Human PD‐1 (Leu25 ‐ Gln167).|
|別名||hPD-l; CD279; PD-1; Protein PD-1; CD antigen CD279; PD1; hSLE1; SLEB2; Programmed cell death protein 1; hPD-1|
|应用说明||IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|纯化||Purification with Protein A.|
|缓冲液||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|抗菌剂||0.01% Sodium azide|
|稳定剂||40% Glycerol and 0.05% BSA|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||programmed cell death 1|
|背景介绍||CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]|
|生物功能||CD279 / PD-1 is an inhibitory receptor on antigen activated T-cells. It plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.
The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552). [UniProt]
PD-1 antibodies; PD-1 ELISA Kits; PD-1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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The best solution for PD-1/PD-L1 research
Examining CTL/NK-mediated cytotoxicity by ELISA
检测图片 (4) Click the Picture to Zoom In
ARG66243 anti-CD279 / PD-1 antibody [SQab1732] ICC/IF image
Immunofluorescence: 293 cells transfected with PD-1 gene, fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66243 anti-CD279 / PD-1 antibody [SQab1732] (green) at 1:10000 and 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.
ARG66243 anti-CD279 / PD-1 antibody [SQab1732] FACS image
Flow Cytometry: 293 cells transfected with PD-1 gene. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66243 anti-CD279 / PD-1 antibody [SQab1732] (blue) at 1:200 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.
ARG66243 anti-CD279 / PD-1 antibody [SQab1732] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung tonsil tissue stained with ARG66243 anti-CD279 / PD-1 antibody [SQab1732] at 1:200. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
ARG66243 anti-CD279 / PD-1 antibody [SQab1732] IP image
Immunoprecipitation: 0.2 mg of 293 whole cell lysate (transfected with PD-1 gene) was immunoprecipitated (1:50 dilution) by ARG66243 anti-CD279 / PD-1 antibody [SQab1732] and then stained with anti-CD279 / PD-1 antibody.
Lane 1: Immunoprecipitation in 293 whole cell lysate (transfected with PD-1 gene)
Lane 2: PBS instead of Primary Ab in 293 whole cell lysate (transfected with PD-1 gene)
Lane 3: 293 whole cell lysate (transfected with PD-1 gene), 2 µg (input)