ARG11039
anti-CD274 / PD-L1 antibody [12B4]
anti-CD274 / PD-L1 antibody [12B4] for Western blot,Sandwich ELISA and Human
概述
| 产品描述 | Mouse Monoclonal antibody [12B4] recognizes CD274 / PD-L1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB, sELISA |
| 特异性 | Reactive with human programmed death ligand 1 (PD-L1). |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 12B4 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD274 / PD-L1 |
| 抗原物种 | Human |
| 抗原 | Human cell expressed recombinant PD-L1 (Phe19-Arg238) with poly-histidine tag at C-terminus. |
| 偶联标记 | Un-conjugated |
| 別名 | Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1 |
应用说明
| 应用建议 |
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| 应用说明 | sELISA: Recommended pairs (capture - detection): ARG11040 anti-CD274 / PD-L1 antibody [15G3] - ARG11039 anti-CD274 / PD-L1 antibody [12B4] * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.0) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD274 |
| 全名 | CD274 molecule |
| 背景介绍 | CD274 / PD-L1 is an immune inhibitory receptor ligand. It is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| 生物功能 | CD274 / PD-L1 plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [UniProt] |
| 细胞定位 | Cell membrane and Endomembrane system. |
| 产品亮点 | Related products: PD-L1 antibodies; PD-L1 ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: The best solution for PD-1/PD-L1 research Examining CTL/NK-mediated cytotoxicity by ELISA |
| 预测分子量 | 33 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG11039 anti-CD274 / PD-L1 antibody [12B4] WB image
Western blot: 1 ng of recombinant Human PD-1 stained with ARG11039 anti-CD274 / PD-L1 antibody [12B4].
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ARG11039 anti-CD274 / PD-L1 antibody [12B4] standard curve image
ARG11039 anti-CD274 / PD-L1 antibody [12B4] results of a typical standard run with optical density reading at 450 nm.
