ARG41640
anti-CD272 / BTLA antibody
anti-CD272 / BTLA antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD272 / BTLA |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD272 / BTLA |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human CD272 / BTLA. (QSNLIESHSTTLYVTDVKSASERPSKDEMASRPWLLYRL) |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD272; BTLA1; B- and T-lymphocyte-associated protein; B- and T-lymphocyte attenuator; CD272 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 33 kDa (nonglycosylated); ~ 65 kDa (glycosylated) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BTLA |
| 全名 | B and T lymphocyte associated |
| 背景介绍 | This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011] |
| 生物功能 | Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response. [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11. N-glycosylated. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG41640 anti-CD272 / BTLA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41640 anti-CD272 / BTLA antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41640 anti-CD272 / BTLA antibody WB image
Western blot: 50 µg of samples under reducing conditions. HEK293, Jurkat, CCRF-CEM and Mouse thymus lysates stained with ARG41640 anti-CD272 / BTLA antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41640 anti-CD272 / BTLA antibody FACS image
Flow Cytometry: THP-1 cells were blocked with 10% normal Goat serum and then stained with ARG41640 anti-CD272 / BTLA antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41640 anti-CD272 / BTLA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41640 anti-CD272 / BTLA antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41640 anti-CD272 / BTLA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41640 anti-CD272 / BTLA antibody at 1 µg/ml dilution, overnight at 4°C.
