ARG10588
anti-CD230 / Prion protein antibody [T16-R]
anti-CD230 / Prion protein antibody [T16-R] for Western blot and Human,Mouse,Rat,Bovine,Sheep
概述
| 产品描述 | Rabbit Monoclonal antibody [T16-R] recognizes CD230 / Prion protein |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov, Sheep |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | T16-R |
| 同位型 | IgG |
| 靶点名称 | CD230 / Prion protein |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the N-terminus of Human prion protein |
| 偶联标记 | Un-conjugated |
| 別名 | GSS; PrPc; PrP33-35C; PrP27-30; Alternative prion protein; p27-30; CJD; ASCR; CD230; PrP; PRIP; KURU; AltPrP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
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| 缓冲液 | 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 10 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | PRNP |
| 全名 | prion protein |
| 背景介绍 | The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
| 预测分子量 | 9 kDa |
| 翻译后修饰 | The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion. Isoform 2 is sumoylated with SUMO1. |
检测图片 (2) Click the Picture to Zoom In
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ARG10588 anti-CD230 / Prion protein antibody [T16-R] WB image
Western blot: 200 µg of A) Mouse brain extract, B) HEK293 cells producing recombinant Human prion protein, and C) HEK293 cells transfected with empty vector stained with ARG10588 anti-CD230 / Prion protein antibody [T16-R].
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ARG10588 anti-CD230 / Prion protein antibody [T16-R] WB image
Western blot: 30 µg of A) PrP-KO CF-10 cells, B) Mouse hippocampus, C) HEK293 cells, transfected with Mouse prion protein bearing 3F4 epitope, and D) 30 ng of recombinant MoPrP stained with ARG10588 anti-CD230 / Prion protein antibody [T16-R].
Performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada.
