ARG53798
anti-CD230 / Prion protein antibody [EM-20]
anti-CD230 / Prion protein antibody [EM-20] for Western blot and Human
Developmental Biology antibody; Neuroscience antibody
概述
| 产品描述 | Mouse Monoclonal antibody [EM-20] recognizes CD230 / Prion protein |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | The clone EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | EM-20 |
| 同位型 | IgG2a |
| 靶点名称 | CD230 / Prion protein |
| 抗原物种 | Human |
| 抗原 | Recombinant human prion protein |
| 偶联标记 | Un-conjugated |
| 別名 | GSS; PrPc; PrP33-35C; PrP27-30; Alternative prion protein; p27-30; CJD; ASCR; CD230; PrP; PRIP; KURU; AltPrP |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Non-reducing condition are essential. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PRNP |
| 全名 | prion protein |
| 背景介绍 | CD230 / Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K. |
| 研究领域 | Developmental Biology antibody; Neuroscience antibody |
| 预测分子量 | 9 kDa |
| 翻译后修饰 | The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion. Isoform 2 is sumoylated with SUMO1. |
检测图片 (1) Click the Picture to Zoom In
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ARG53798 anti-CD230 / Prion protein antibody [EM-20] WB image
Western blot: 1,2. Creutzfeld-Jakob disease (CJD) negative Human brain tissue lysate 3,4. CJD positive Human brain tissue lysate stained with ARG53798 anti-CD230 / Prion protein antibody [EM-20].
CJD positive patient has proteinase K resistent prion protein. Lane 1,4: Samples with proteinase K treatment. Lane 2,3: Samples without proteinase K treatment.
