ARG62779
anti-CD229 antibody [HLy9.25]
anti-CD229 antibody [HLy9.25] for Flow cytometry,Functional study,ICC/IF,Immunoprecipitation,Western blot and Human
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [HLy9.25] recognizes CD229 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, ICC/IF, IP, WB |
| 特异性 | The clone HLy9.25 (also known as HLy9.1.25) recognizes CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | HLy9.25 |
| 同位型 | IgG1 |
| 靶点名称 | CD229 |
| 抗原 | CD299-transfected 300.19 pre-B cell line |
| 偶联标记 | Un-conjugated |
| 別名 | Signaling lymphocytic activation molecule 3; T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; hly9; Lymphocyte antigen 9; mLY9; CD229; SLAMF3; CD antigen CD229; SLAM family member 3 |
应用说明
| 应用建议 |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Functional studies: Regulation of activation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||
| 阳性对照 | FACS: Peripheral blood lymphocytes |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LY9 |
| 全名 | lymphocyte antigen 9 |
| 背景介绍 | CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation. |
| 生物功能 | May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 72 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG62779 anti-CD229 antibody [HLy9.25] FACS image
Flow Cytometry: Human peripheral blood stained with ARG62779 anti-CD229 antibody [HLy9.25] at 16 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
-
ARG62779 anti-CD229 antibody [HLy9.25] FACS image
Flow Cytometry: Separation of human CD229 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed), Human peripheral whole blood stained with ARG62779 anti-CD229 antibody [HLy9.25] at 16 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse.
