ARG43308
anti-CD226 antibody
anti-CD226 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD226 |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | FACS, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD226 |
| 抗原物种 | Mouse |
| 抗原 | Recombinant protein corresponding to E31-L333 of Mouse CD226. |
| 偶联标记 | Un-conjugated |
| 別名 | TLiSA1; CD antigen CD226; PTA1; CD226 antigen; DNAM-1; DNAX accessory molecule 1; DNAM1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD226 |
| 全名 | CD226 molecule |
| 背景介绍 | This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| 生物功能 | Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell (PubMed:8673704). Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding (PubMed:26755705). [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 39 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG43308 anti-CD226 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43308 anti-CD226 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43308 anti-CD226 antibody FACS image
Flow Cytometry: NRK cells were blocked with 10% normal goat serum and then stained with ARG43308 anti-CD226 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43308 anti-CD226 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung lymphaden tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43308 anti-CD226 antibody at 2 µg/ml dilution, overnight at 4°C.
