ARG41732
anti-CD22 antibody
anti-CD22 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Developmental Biology antibody; Immune System antibody; Immature B Cell Marker antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD22 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD22 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 696-724 of Human CD22. (LAILILAICGLKLQRRWKRTQSQQGLQEN) |
| 偶联标记 | Un-conjugated |
| 別名 | B-lymphocyte cell adhesion molecule; B-cell receptor CD22; T-cell surface antigen Leu-14; BL-CAM; SIGLEC-2; Sialic acid-binding Ig-like lectin 2; Siglec-2; CD antigen CD22; SIGLEC2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 135 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD22 |
| 全名 | CD22 molecule |
| 生物功能 | Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Immune System antibody; Immature B Cell Marker antibody |
| 预测分子量 | 95 kDa |
| 翻译后修饰 | Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. Phosphorylated on tyrosine residues by LYN. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG41732 anti-CD22 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG41732 anti-CD22 antibody.
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ARG41732 anti-CD22 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Raji, Rat thymus and Mouse thymus lysates stained with ARG41732 anti-CD22 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41732 anti-CD22 antibody FACS image
Flow Cytometry: Raji cells were blocked with 10% normal goat serum and then stained with ARG41732 anti-CD22 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
