ARG66759
anti-CD2 antibody [SQab20192]
anti-CD2 antibody [SQab20192] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab20192] recognizes CD2 |
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| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab20192 |
| 同位型 | IgG |
| 靶点名称 | CD2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the N-terminus of Human CD2. |
| 偶联标记 | Un-conjugated |
| 別名 | T-cell surface antigen T11/Leu-5; LFA-3 receptor; T-cell surface antigen CD2; SRBC; Erythrocyte receptor; CD antigen CD2; T11; Rosette receptor; LFA-2 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | WB: Jurkat IHC-P: T-cell lymphoma tissue |
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| 实际分子量 | 43 ~ 47 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD2 |
| 全名 | CD2 molecule |
| 背景介绍 | The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene. [provided by RefSeq, Jun 2016] |
| 生物功能 | CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 39 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG66759 anti-CD2 antibody [SQab20192] WB image (Customer's Feedback)
Western blot: 10 µg of Jurkat cell lysate stained with ARG66759 anti-CD2 antibody [SQab20192] at 1:1000 dilution.
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ARG66759 anti-CD2 antibody [SQab20192] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human T-cell lymphoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66759 anti-CD2 antibody [SQab20192] at 18°C - 25°C for 30 minutes.
客户反馈
Excellent
anti-CD2 antibody [SQab20192]
Application：WB
Sample：Jurkat
Sample Loading Amount：10 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
