ARG65544
anti-CD195 / CCR5 antibody [T21/8]
anti-CD195 / CCR5 antibody [T21/8] for ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [T21/8] recognizes CD195 / CCR5 |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, ICC/IF, IP, WB |
| 特异性 | The mouse monoclonal antibody T21/8 recognizes the Nteminus of CD195, an approximately 45 kDa Gprotein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | T21/8 |
| 同位型 | IgG1 |
| 靶点名称 | CD195 / CCR5 |
| 抗原 | CCR5 peptide (Met1-Lys22) KLH conjugate |
| 偶联标记 | Un-conjugated |
| 別名 | CHEMR13; CD195; C-C chemokine receptor type 5; CKR-5; CCCKR5; CCR-5; CD antigen CD195; CKR5; CC-CKR-5; IDDM22; CCR5; CMKBR5; C-C CKR-5; HIV-1 fusion coreceptor |
应用说明
| 应用建议 |
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| 应用说明 | WB: Do not boil cell lysates prior to SDS-PAGE. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCR5 |
| 全名 | chemokine (C-C motif) receptor 5 (gene/pseudogene) |
| 背景介绍 | CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120. |
| 生物功能 | Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 41 kDa |
| 翻译后修饰 | Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4. O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen. Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry. Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. |
检测图片 (2) Click the Picture to Zoom In
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ARG65544 anti-CD195 / CCR5 antibody [T21/8] FACS image
Flow Cytometry: Human peripheral blood stained with ARG65544 anti-CD195 / CCR5 antibody [T21/8] at 3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65544 anti-CD195 / CCR5 antibody [T21/8] FACS image
Flow Cytometry: Separation of human CD195 positive lymphocytes (red-filled) from CD195 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65544 anti-CD195 / CCR5 antibody [T21/8] at 3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
