ARG54318
anti-CD195 / CCR5 antibody
anti-CD195 / CCR5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD195 / CCR5 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD195 / CCR5 |
| 抗原物种 | Human |
| 抗原 | Peptide corresponding to aa 6-20 of human CCR5. |
| 偶联标记 | Un-conjugated |
| 別名 | CHEMR13; CD195; C-C chemokine receptor type 5; CKR-5; CCCKR5; CCR-5; CD antigen CD195; CKR5; CC-CKR-5; IDDM22; CCR5; CMKBR5; C-C CKR-5; HIV-1 fusion coreceptor |
应用说明
| 应用建议 |
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| 应用说明 | Western blot: use at 1:1,000 - 1:2,000 dilution. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | THP-1 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunoaffinity chroma-tography |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCR5 |
| 全名 | chemokine (C-C motif) receptor 5 (gene/pseudogene) |
| 背景介绍 | Human immunodeficiency virus (HIV) and related viruses require coreceptors in addition to CD4 to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, Bonzo, GPR1, and V28, have been identified as HIV coreceptors. Among them, CCR5 is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains. CCR5 is required for infection by HIV-1, HIV-2, and SIV. The β-chemokines RANTES, MIP-1α, and MIP-1β are the ligands for CCR5 and prevent infection by macrophage-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV binding sites. Messenger RNA for CCR5 is expressed in lymphoid cells and tissues. |
| 生物功能 | Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 41 kDa |
| 翻译后修饰 | Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4. O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen. Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry. Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. |
检测图片 (2) Click the Picture to Zoom In
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ARG54318 anti-CD195 / CCR5 antibody IHC-P image
Immunohistochemistry: Human lymph node stained with ARG54318 anti-CD195 / CCR5 antibody at 20 μg/ml dilution.
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ARG54318 anti-CD195 / CCR5 antibody IHC-P image
Immunohistochemistry: Human lymph node stained with ARG54318 anti-CD195 / CCR5 antibody at 2.5 μg/ml dilution.
