ARG56009
anti-CD195 / CCR5 antibody [12D1]
anti-CD195 / CCR5 antibody [12D1] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Mouse Monoclonal antibody [12D1] recognizes CD195 / CCR5 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 12D1 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | CD195 / CCR5 |
| 抗原物种 | Human |
| 抗原 | Human native CD195 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | CHEMR13; CD195; C-C chemokine receptor type 5; CKR-5; CCCKR5; CCR-5; CD antigen CD195; CKR5; CC-CKR-5; IDDM22; CCR5; CMKBR5; C-C CKR-5; HIV-1 fusion coreceptor |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 20 min, followed by cooling at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCR5 |
| 全名 | chemokine (C-C motif) receptor 5 (gene/pseudogene) |
| 背景介绍 | This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2015] |
| 生物功能 | Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. [UniProt] |
| 细胞定位 | Cell surface |
| 预测分子量 | 41 kDa |
| 翻译后修饰 | Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4. O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen. Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry. Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. |
检测图片 (5) Click the Picture to Zoom In
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ARG56009 anti-CD195 / CCR5 antibody [12D1] ICC/IF image
Immunofluorescence: PFA-fixed HeLa cells stained with ARG56009 anti-CD195 / CCR5 antibody [12D1] (green).
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ARG56009 anti-CD195 / CCR5 antibody [12D1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human stomach tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 20 min, followed by cooling at RT. The tissue section was stained with ARG56009 anti-CD195 / CCR5 antibody [12D1].
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ARG56009 anti-CD195 / CCR5 antibody [12D1] FACS image
Flow Cytometry: PFA-fixed HeLa cells stained with ARG56009 anti-CD195 / CCR5 antibody [12D1] (blue); Isotype control (red).
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ARG56009 anti-CD195 / CCR5 antibody [12D1] FACS image
Flow Cytometry: MCF7 cells stained with ARG56009 anti-CD195 / CCR5 antibody [12D1] (blue); Isotype control (red).
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ARG56009 anti-CD195 / CCR5 antibody [12D1] FACS image
Flow Cytometry: U-87 MG cells stained with ARG56009 anti-CD195 / CCR5 antibody [12D1] (blue); Isotype control (red).
