ARG66321

anti-CD19 antibody [SQab1869]

anti-CD19 antibody [SQab1869] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; B cell Marker antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody; Immature B Cell Marker antibody; Follicular dendritic cells antibody

概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab1869] recognizes CD19
反应物种 Hu
应用 FACS, ICC/IF, IHC-P, IP, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab1869
同位型 IgG
靶点名称 CD19
抗原物种 Human
抗原 Synthetic peptide around the C-terminus of CD19.
偶联标记 Un-conjugated
別名 Differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD antigen CD19; CVID3

应用说明

应用建议
应用 推荐稀释比
FACS1:10
ICC/IF1:10 - 1:50
IHC-P1:200 - 1:400
IP1:50
WB1:2000 - 1:10000
应用说明 IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 930 Human CD19

Swiss-port # P15391 Human B-lymphocyte antigen CD19

基因名称 CD19
全名 CD19 molecule
背景介绍 CD19: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
生物功能 CD19 functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701). [UniProt]
产品亮点 Related news:
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MyD88 L265P antibody for lymphoma research;

Related products:
CD19 antibodies; CD19 ELISA Kits; CD19 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; B cell Marker antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody; Immature B Cell Marker antibody; Follicular dendritic cells antibody
预测分子量 61 kDa
翻译后修饰 Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG66321 anti-CD19 antibody [SQab1869] ICC/IF image

    Immunofluorescence: Raji cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66321 anti-CD19 antibody [SQab1869] (green) at 1:50 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66321 anti-CD19 antibody [SQab1869] FACS image

    Flow Cytometry: Ramos cells were fixed with 4% paraformaldehyde for 10 mins. The cells were stained with ARG66321 anti-CD19 antibody [SQab1869] (blue) at 1:10 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG66321 anti-CD19 antibody [SQab1869] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded spleen tissue stained with ARG66321 anti-CD19 antibody [SQab1869] at 1:400 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG66321 anti-CD19 antibody [SQab1869] WB image

    Western blot: 10 µg of Ramos, Daudi and Raji cell lysates stained with ARG66321 anti-CD19 antibody [SQab1869] at 1:2000 dilution.

  • ARG66321 anti-CD19 antibody [SQab1869] IP image

    Immunoprecipitation: 0.4 mg of Ramos lysate immunoprecipitated (1:50) and stained with ARG66321 anti-CD19 antibody [SQab1869]. 1) ARG66321 IP in Ramos whole cell lysate, 2) Rabbit IgG instead of ARG66321 in Ramos whole cell lysate, and 3) Ramos whole cell lysate, 10 µg (input).