ARG53777
anti-CD177 antibody [MEM-166] (PE)
anti-CD177 antibody [MEM-166] (PE) for Flow cytometry and Human,Primates
Immune System antibody
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [MEM-166] recognizes CD177 |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | FACS |
| 特异性 | The clone MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes. HLDA VI; WS Code M M17 HLDA VI; WS Code BP 309 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-166 |
| 同位型 | IgG1 |
| 靶点名称 | CD177 |
| 抗原物种 | Human |
| 抗原 | Human granulocytes |
| 偶联标记 | PE |
| 別名 | NB1 glycoprotein; PRV-1; PRV1; NB1; CD177 antigen; Human neutrophil alloantigen 2a; NB1 GP; HNA2A; Polycythemia rubra vera protein 1; CD antigen CD177; HNA-2a |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD177 |
| 全名 | CD177 molecule |
| 背景介绍 | CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN). |
| 研究领域 | Immune System antibody |
| 预测分子量 | 46 kDa |
| 翻译后修饰 | N-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). |
克隆号文献
Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177.
WB, FACS / Human
The Thr224Asn mutation in the VPS45 gene is associated with the congenital neutropenia and primary myelofibrosis of infancy.
IHC-P / Human
Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.
Decreased CXCR1 and CXCR2 expression on neutrophils in anti-neutrophil cytoplasmic autoantibody-associated vasculitides potentially increases neutrophil adhesion and impairs migration.
FACS / Human
CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses.
FACS / Human
