ARG56138

anti-CD163 antibody

anti-CD163 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description

Rabbit Polyclonal antibody recognizes CD163

Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name CD163
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide from Human CD163.
Conjugation Un-conjugated
Full Name CD163 molecule
Alternate Names sCD163; M130; Scavenger receptor cysteine-rich type 1 protein M130; MM130; CD antigen CD163; Hemoglobin scavenger receptor

Application Instructions

Application Suggestion
Application Dilution
FACS1:20 - 1:100
IHC-P1:100 - 1:500
WB1:100 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 130 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.01M TBS (pH 7.4), 0.03% Proclin-300, 1% BSA and 50% Glycerol
Preservative 0.03% Proclin-300
Stabilizer 1% BSA and 50% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 9332 Human CD163

GeneID: 93671 Mouse CD163

Swiss-port # Q2VLH6 Mouse Scavenger receptor cysteine-rich type 1 protein M130

Swiss-port # Q86VB7 Human Scavenger receptor cysteine-rich type 1 protein M130

Gene Symbol CD163
Gene Full Name CD163 molecule
Background The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Function Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. [UniProt]
Cellular Localization Soluble CD163: Secreted.
PTM A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated.

Images (6) Click the Picture to Zoom In

  • ARG56138 anti-CD163 antibody FACS image

    Flow Cytometry: Mouse splenocytes stained with FITC-Conjugated ARG56138 anti-CD163 antibody at 1:10 dilution for 30 min (green). Controls: unstained cells (blue) and isotype control (orange).

  • ARG56138 anti-CD163 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin embedded Rat colon tissue stained with ARG56138 anti-CD163 antibody.

  • ARG56138 anti-CD163 antibody WB image

    Western blot: 1) L1 Rat brain and 2) L2 Mouse uterus lysates stained with ARG56138 anti-CD163 antibody at 1:200 dilution (4˚C).

  • ARG56138 anti-CD163 antibody FACS image

    Flow Cytometry: SC96 cells stained with ALEXA FLUOR® 647-Conjugated ARG56138 anti-CD163 antibody at 1:20 for 30 min (green). Controls: control cells (blue) and isotype control (orange).

  • ARG56138 anti-CD163 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin embedded Mouse intestine stained with ARG56138 anti-CD163 antibody at 1:200 dilution.

  • ARG56138 anti-CD163 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin embedded Human testis tissue stained with ARG56138 anti-CD163 antibody at 1:100 (40 min, 37°C). DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative.