ARG42248
anti-CD16 antibody [3G8] (PerCP)
anti-CD16 antibody [3G8] (PerCP) for Flow cytometry and Human,Primates
概述
| 产品描述 | PerCP-conjugated Mouse Monoclonal antibody [3G8] recognizes CD16 |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | FACS |
| 特异性 | The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils). |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3G8 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD16 |
| 抗原物种 | Human |
| 抗原 | Human neutrophils. |
| 偶联标记 | PerCP |
| 別名 | FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS and 15 mM Sodium azide. |
| 抗菌剂 | 15 mM Sodium azide |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08637 Human Low affinity immunoglobulin gamma Fc region receptor III-A |
|---|---|
| 基因名称 | FCGR3A |
| 全名 | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
| 背景介绍 | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Secreted. Note=Exists also as a soluble receptor. [UniProt] |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms. The soluble form is produced by a proteolytic cleavage. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
-
ARG42248 anti-CD16 antibody [3G8] (PerCP) FACS image
Flow Cytometry: Human peripheral blood stained with ARG42248 anti-CD16 antibody [3G8] (PerCP) and anti-CD3 antibody (FITC).
