ARG23148
anti-CD152 / CTLA4 antibody [WKH203]
anti-CD152 / CTLA4 antibody [WKH203] for ELISA,Flow cytometry,Western blot and Rat
概述
| 产品描述 | Mouse Monoclonal antibody [WKH203] recognizes CD152 / CTLA4 Mouse anti Rat CD152 antibody, clone WKH203 recognizes rat CD152, also known as cytoxic-T-lymphocyte antigen-4 (CTLA-4), which is similar in structure to CD28 and also binds ligands CD80 and CD86. CD152 is expressed by activated T-lymphocytes and studies also suggest expression by regulatory T-lymphocytes (Lin and Hunig 2003). |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA, FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | WKH203 |
| 同位型 | IgG1 |
| 靶点名称 | CD152 / CTLA4 |
| 抗原物种 | Rat |
| 抗原 | Purified rCTLA-4hIg fusion protein. |
| 偶联标记 | Un-conjugated |
| 別名 | GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | Ctla4 |
|---|---|
| 全名 | cytotoxic T-lymphocyte-associated protein 4 |
| 背景介绍 | This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008] |
| 生物功能 | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG23148 anti-CD152 / CTLA4 antibody [WKH203] FACS image
Flow Cytometry: Rat CD152 transfected cell line using ARG23148 anti-CD152 / CTLA4 antibody [WKH203] following permeabilisation.
