ARG66927
anti-CD152 / CTLA4 antibody [SQab22255]
anti-CD152 / CTLA4 antibody [SQab22255] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab22255] recognizes CD152 / CTLA4 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab22255 |
| 同位型 | IgG |
| 靶点名称 | CD152 / CTLA4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within Human CTLA-4. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Cytotoxic T-lymphocyte protein 4 |
| 別名 | GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human lymphoma tissue |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05%BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTLA4 |
| 全名 | cytotoxic T-lymphocyte-associated protein 4 |
| 背景介绍 | This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008] |
| 生物功能 | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt] |
| 细胞定位 | Cell membrane, Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG66927 anti-CD152 / CTLA4 antibody [SQab22255] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human lymphoma tissue stained with ARG66927 anti-CD152 / CTLA4 antibody [SQab22255]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).
