ARG62735
anti-CD15 antibody [MEM-158]
anti-CD15 antibody [MEM-158] for CyTOF®-candidate,Flow cytometry,Immunoprecipitation and Human
Controls and Markers antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-158] recognizes CD15 |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Bov, Pig, Sheep |
| 应用 | CyTOF®-candidate, FACS, IP |
| 特异性 | The clone MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. HLDA VI; WS Code AS A053 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-158 |
| 同位型 | IgM |
| 靶点名称 | CD15 |
| 抗原物种 | Human |
| 抗原 | Human granulocytes |
| 偶联标记 | Un-conjugated |
| 別名 | LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by precipitation methods and ion exchange chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | TBS (pH 8.0) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FUT4 |
| 全名 | fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) |
| 背景介绍 | CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease. |
| 生物功能 | May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt] |
| 研究领域 | Controls and Markers antibody |
| 预测分子量 | 59 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG62735 anti-CD15 antibody [MEM-158] FACS image
Flow Cytometry: Human peripheral blood stained with ARG62735 anti-CD15 antibody [MEM-158] at 0.3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62735 anti-CD15 antibody [MEM-158] FACS image
Flow Cytometry: Separation of human neutrophil granulocytes (red-filled) from CD15 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62735 anti-CD15 antibody [MEM-158] at 0.3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62735 anti-CD15 antibody [MEM-158] CyTOF image
CyTOF: Human peripheral blood cells (after ammonium chloride red blood cell lysis) stained with ARG62735 anti-CD15 antibody [MEM-158] (173Yb). Singlet cells were gated for data analysis.
