ARG65504
anti-CD148 / DEP1 antibody [MEM-CD148 / 05]
anti-CD148 / DEP1 antibody [MEM-CD148 / 05] for Flow cytometry and Human
Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-CD148/05] recognizes CD148 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone MEM-CD148/05 recognizes CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-CD148/05 |
| 同位型 | IgG2b |
| 靶点名称 | CD148 / DEP1 |
| 抗原物种 | Human |
| 抗原 | Human recombinant CD148 (amino acids 1-444) |
| 偶联标记 | Un-conjugated |
| 別名 | R-PTP-J; Density-enhanced phosphatase 1; R-PTP-ETA; DEP-1; SCC1; DEP1; CD antigen CD148; Receptor-type tyrosine-protein phosphatase eta; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; EC 3.1.3.48; HPTPeta; HPTP eta; R-PTP-eta; CD148 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q12913 Human Receptor-type tyrosine-protein phosphatase eta |
|---|---|
| 基因名称 | PTPRJ |
| 全名 | protein tyrosine phosphatase, receptor type, J |
| 背景介绍 | CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes. |
| 生物功能 | Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 146 kDa |
| 翻译后修饰 | N- and O-glycosylated. |
