ARG41554
anti-CD147 antibody
anti-CD147 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD147 |
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| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD147 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to E138-A323 of Human CD147. |
| 偶联标记 | Un-conjugated |
| 別名 | OK; Tumor cell-derived collagenase stimulatory factor; Leukocyte activation antigen M6; TCSF; Extracellular matrix metalloproteinase inducer; OK blood group antigen; CD147; Basigin; M6; EMMPRIN; Collagenase stimulatory factor; CD antigen CD147; 5F7 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 35 kDa (nonglycosylated); ~ 50 kDa (glycosylated) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BSG |
| 全名 | basigin (Ok blood group) |
| 背景介绍 | The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In spermatozoa, localized on the principal piece of caput and in the middle piece during transit in the corpus and cauda epididymides (By similarity). [UniProt] |
| 预测分子量 | 42 kDa |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (8) Click the Picture to Zoom In
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ARG41554 anti-CD147 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG41554 anti-CD147 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG41554 anti-CD147 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41554 anti-CD147 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41554 anti-CD147 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Jurkat and U2OS whole cell lysates stained with ARG41554 anti-CD147 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41554 anti-CD147 antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal Goat serum and then stained with ARG41554 anti-CD147 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41554 anti-CD147 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41554 anti-CD147 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41554 anti-CD147 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intetsinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41554 anti-CD147 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41554 anti-CD147 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41554 anti-CD147 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41554 anti-CD147 antibody FACS image
Flow Cytometry: HeLa cells were blocked with 10% normal Goat serum and then stained with ARG41554 anti-CD147 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
