ARG23485
anti-CD14 antibody [VPM65]
anti-CD14 antibody [VPM65] for ELISA,Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Bovine,Goat,Sheep
Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
概述
| 产品描述 | Mouse Monoclonal antibody [VPM65] recognizes CD14 Mouse Anti Sheep CD14 antibody, clone VPM65 recognizes Ovine CD14, a GPI-anchored 55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes.CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, which has been shown to upregulate the cell surface expression of adhesion molecules. |
|---|---|
| 反应物种 | Bov, Goat, Sheep |
| 应用 | ELISA, FACS, IHC-Fr, IP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | VPM65 |
| 同位型 | IgG1 |
| 靶点名称 | CD14 |
| 抗原物种 | Sheep |
| 抗原 | Ovine macrophages |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or cells or 100 µl whole blood * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q95122 Bovine Monocyte differentiation antigen CD14 |
|---|---|
| 基因名称 | CD14 |
| 全名 | CD14 molecule |
| 背景介绍 | The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010] |
| 生物功能 | In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG23485 anti-CD14 antibody [VPM65] FACS image
Flow Cytometry: Sheep peripheral blood granulocytes stained with ARG23485 anti-CD14 antibody [VPM65] followed by Goat anti Mouse IgG (FITC).
