ARG22939
anti-CD14 antibody [TÜK4]
anti-CD14 antibody [TÜK4] for ELISA,Flow cytometry,Western blot and Human,Bovine,Cat,Dog,Goat,Pig,Rabbit,Sheep
Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
概述
| 产品描述 | Mouse Monoclonal antibody [TÜK4] recognizes CD14 Mouse anti human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen. CD14 is a 55 KDa glycoprotein that contains multiple leucin-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage (Simmons et al. 1989) but a soluble form of CD14 also exists (Bazil et al. 1986).CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Pugin et al. 1994, Dziarski et al. 1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria.Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose âdependent manner (Yang et al. 2003). Arigo recommend the use of the anti-human CD14 antibody, Low Endotoxin format for this purpose. |
|---|---|
| 反应物种 | Hu, Bov, Cat, Dog, Goat, Pig, Rb, Sheep |
| 应用 | ELISA, FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | TÜK4 |
| 同位型 | IgG2a |
| 靶点名称 | CD14 |
| 抗原物种 | Human |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 5ul of the suggested working dilution to label 10^6 cells or 100 µl whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD14 |
| 全名 | CD14 molecule |
| 背景介绍 | The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010] |
| 生物功能 | In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
