ARG62727
anti-CD14 antibody [B-A8]
anti-CD14 antibody [B-A8] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
概述
| 产品描述 | Mouse Monoclonal antibody [B-A8] recognizes CD14 |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, ICC/IF, IHC-P |
| 特异性 | The clone B-A8 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | B-A8 |
| 同位型 | IgG1 |
| 靶点名称 | CD14 |
| 抗原物种 | Human |
| 抗原 | Human monocytes |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14 |
应用说明
| 应用建议 |
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| 应用说明 | ELISA: The clone B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (ARG62725). IHC-P: Heat retrieval of antigen is recommeded. Staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | IHC-P: PML brain section |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by protein A |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08571 Human Monocyte differentiation antigen CD14 |
|---|---|
| 基因名称 | CD14 |
| 全名 | CD14 molecule |
| 背景介绍 | CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes. |
| 生物功能 | In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
检测图片 (1) Click the Picture to Zoom In
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ARG62727 anti-CD14 antibody [B-A8] FACS image
Flow Cytometry: Separation of human monocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62727 anti-CD14 antibody [B-A8] at 1.7 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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