ARG58839
anti-CD127 / IL7R antibody
anti-CD127 / IL7R antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD127 / IL7R |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Rat |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD127 / IL7R |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 278-315 of Human CD127 (DHKKTLEHLCKKPRKNLNVSFNPESFLDCQIHRVDDIQ). |
| 偶联标记 | Un-conjugated |
| 別名 | ILRA; CD127; IL-7R subunit alpha; IL-7R-alpha; IL7RA; CD antigen CD127; Interleukin-7 receptor subunit alpha; CDW127; CDw127; IL-7 receptor subunit alpha; IL-7RA |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P16871 Human Interleukin-7 receptor subunit alpha |
|---|---|
| 基因名称 | IL7R |
| 全名 | interleukin 7 receptor |
| 背景介绍 | The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2014] |
| 生物功能 | Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58839 anti-CD127 / IL7R antibody WB image
Western blot: 22RV1 cell lysate stained with ARG58839 anti-CD127 / IL7R antibody at 0.5 µg/ml dilution.
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ARG58839 anti-CD127 / IL7R antibody FACS image
Flow Cytometry: U2OS cells were blocked with 10% normal goat serum and then stained with ARG58839 anti-CD127 / IL7R antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58839 anti-CD127 / IL7R antibody FACS image
Flow Cytometry: U87 cells were blocked with 10% normal goat serum and then stained with ARG58839 anti-CD127 / IL7R antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
