ARG22728
anti-CD11d antibody [CA11.8H2]
anti-CD11d antibody [CA11.8H2] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Dog
概述
| 产品描述 | Mouse Monoclonal antibody [CA11.8H2] recognizes CD11d This antibody recognizes Canine CD11d cell surface antigen, also known as integrin alpha d subunit. The alpha d integrin (CD11d) subunit is expressed as a heterodimer with beta 2 integrin (CD18). CD11d is expressed by a sub-population of macrophages, some CD8 positive T cells and by gamma/delta T cells in the splenic red pulp. Over expression of CD11d is noted in histiocytic disorders including hemophagocytic sarcoma, described as a proliferative disorder specifically of CD11+ macrophages (Moore. et al. 2006) with macrophages demonstrating marked erythrocytic phagocytosis. Previously, Canine histiocytic sarcoma were described as originating entirely from interstitial dendritic cells. Cells from animals with Canine hepatosplenic T cell lymphoma (HTSL) strongly express CD11d on malignant cells. Dogs normally demonstrate strong positivity for CD11d on large granular lymphocytes, macrophages and T cells in the splenic red pulp. CD11+ γ/δ T cells constitute ~30% red pulp T cells despite their rarity in peripheral blood, suggesting a splenic red pulp origin of HTSL (Fry et al. 2003). |
|---|---|
| 反应物种 | Dog |
| 应用 | FACS, IHC-Fr, IP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CA11.8H2 |
| 同位型 | IgG1 |
| 靶点名称 | CD11d |
| 抗原物种 | Dog |
| 抗原 | Affinity purified CD11 antigen from canine spleen. |
| 偶联标记 | Un-conjugated |
| 別名 | Integrin alpha-D; CD11 antigen-like family member D; ADB2; Leukointegrin alpha D; CD11D; CD antigen CD11d |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | ITGAD |
|---|---|
| 全名 | integrin, alpha D |
| 生物功能 | Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. May play a role in the atherosclerotic process such as clearing lipoproteins from plaques and in phagocytosis of blood-borne pathogens, particulate matter, and senescent erythrocytes from the blood. [UniProt] |
| 预测分子量 | 127 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG22728 anti-CD11d antibody [CA11.8H2] FACS image
Flow Cytometry: Canine peripheral blood lymphocytes stained with ARG22728 anti-CD11d antibody [CA11.8H2] followed by Goat anti-Mouse IgG (H/L) (FITC).
