ARG65493
anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free)
anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) for Flow cytometry,Functional study,Immunoprecipitation and Human
Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Azide free Mouse Monoclonal antibody [MEM-25] recognizes CD11a / LFA1 alpha |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IP |
| 特异性 | The clone MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. HLDA IV; WS Code NL 209 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-25 |
| 同位型 | IgG1 |
| 靶点名称 | CD11a / LFA1 alpha |
| 抗原 | Leukocytes from a pacient suffering from a LGL-type leukaemia. |
| 偶联标记 | Un-conjugated |
| 別名 | Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IP: Excellent antibody for immunoaffinity purification of LFA-1 complex. Functional studies: The clone MEM-25 partially blocks binding of LFA-1 complex to ICAM-1. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGAL |
| 全名 | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
| 背景介绍 | CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. |
| 生物功能 | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 129 kDa |
| 翻译后修饰 | In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents. |
检测图片 (2) Click the Picture to Zoom In
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ARG65493 anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG65493 anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65493 anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) FACS image
Flow Cytometry: Separation of human monocytes (red-filled) from blood debris (black-dashed). Human peripheral whole blood stained with ARG65493 anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein.
