ARG62707

anti-CD11a / LFA1 alpha antibody [MEM-25]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
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0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [MEM-25] recognizes CD11a / LFA1 alpha
反应物种	Hu
应用	FACS, FuncSt, IP
特异性	The clone MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. HLDA IV; WS Code NL 209
宿主	Mouse
克隆	Monoclonal
克隆号	MEM-25
同位型	IgG1
靶点名称	CD11a / LFA1 alpha
抗原	Leukocytes from a pacient suffering from a LGL-type leukaemia.
偶联标记	Un-conjugated
別名	Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a

应用说明

应用建议

应用	推荐稀释比
FACS	2 µg/ml
FuncSt	Assay-dependent
IP	Assay-dependent

应用说明

IP: Immunoaffinity purification of LFA-1 complex.
Functional studies: The clone MEM-25 partially blocks binding of LFA-1 complex to ICAM-1.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from ascites by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3683 Human ITGAL Swiss-port # P20701 Human Integrin alpha-L
基因名称	ITGAL
全名	integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
背景介绍	CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
生物功能	Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. [UniProt]
研究领域	Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
预测分子量	129 kDa
翻译后修饰	In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents.

检测图片 (2) Click the Picture to Zoom In

ARG62707 anti-CD11a / LFA1 alpha antibody [MEM-25] FACS image

Flow Cytometry: Human peripheral whole blood stained with ARG62707 anti-CD11a / LFA1 alpha antibody [MEM-25] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG62707 anti-CD11a / LFA1 alpha antibody [MEM-25] FACS image

Flow Cytometry: Separation of human monocytes (red-filled) from blood debris (black-dashed). Human peripheral whole blood stained with ARG62707 anti-CD11a / LFA1 alpha antibody [MEM-25] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.