ARG62711
anti-CD11a / LFA1 alpha antibody [M17 / 4] (Functional Grade)
anti-CD11a / LFA1 alpha antibody [M17 / 4] (Functional Grade) for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation and Mouse
Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Functional grade and low endotoxin Rat Monoclonal antibody [M17/4] recognizes CD11a / LFA1 alpha |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, FuncSt, IHC-Fr, IP |
| 特异性 | The clone M17/4 reacts with CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | M17/4 |
| 同位型 | IgG2a |
| 靶点名称 | CD11a / LFA1 alpha |
| 抗原物种 | Mouse |
| 抗原 | C57BL/6 mouse splenic secondary cytotoxic T lymphocytes |
| 偶联标记 | Un-conjugated |
| 別名 | Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a |
应用说明
| 应用建议 |
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| 应用说明 | IHC-Fr: Acetone fixation. Functional studies: Blocking. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | IHC-Fr: Mouse spleen or thymus |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 129 kDa |
| 翻译后修饰 | In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents. |
检测图片 (2) Click the Picture to Zoom In
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ARG62711 anti-CD11a / LFA1 alpha antibody [M17 / 4] (Functional Grade) FACS image
Flow Cytometry: Murine splenocytes stained with ARG62711 anti-CD11a / LFA1 alpha antibody [M17 / 4] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
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ARG62711 anti-CD11a / LFA1 alpha antibody [M17 / 4] (Functional Grade) FACS image
Flow Cytometry: Separation of murine myeloid cells (red-filled) from cellular debris (black-dashed). Murine splenocytes stained with ARG62711 anti-CD11a / LFA1 alpha antibody [M17 / 4] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
