anti-CD117 / c-Kit antibody [SQab20181]
anti-CD117 / c-Kit antibody [SQab20181] for IHC-Formalin-fixed paraffin-embedded sections and Human
|产品描述||Recombinant Rabbit Monoclonal antibody [SQab20181] recognizes CD117 / c-Kit|
|靶点名称||CD117 / c-Kit|
|抗原||Synthetic peptide within aa. 876-976 of Human CD117.|
|別名||PBT; C-Kit; Tyrosine-protein kinase Kit; CD antigen CD117; Mast/stem cell growth factor receptor Kit; CD117; Proto-oncogene c-Kit; SCFR; Piebald trait protein; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; p145 c-kit; EC 18.104.22.168|
|应用说明||IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|阳性对照||Gastrointestinal stromal tumor tissue.|
|纯化||Purification with Protein A.|
|缓冲液||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|抗菌剂||0.01% Sodium azide|
|稳定剂||40% Glycerol and 0.05% BSA|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog|
|背景介绍||This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]|
|生物功能||Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. [UniProt]|
|细胞定位||Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Single-pass type I membrane protein. Isoform 3: Cytoplasm. Note=Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head. [UniProt]|
|翻译后修饰||Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7. [UniProt]
检测图片 (1) Click the Picture to Zoom In
ARG66748 anti-CD117 / c-Kit antibody [SQab20181] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human gastrointestinal stromal tumor tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66748 anti-CD117 / c-Kit antibody [SQab20181] at 18°C - 25°C for 30 minutes.