ARG22999
anti-CD107b / LAMP2 antibody [M3/84]
anti-CD107b / LAMP2 antibody [M3/84] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse
概述
| 产品描述 | Rat Monoclonal antibody [M3/84] recognizes CD107b / LAMP2 Rat anti Mouse CD107b antibody, clone M3/84 recognizes murine CD107b, also known as MAC-3 and LAMP-2. CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between ~92-120 kDa. Studies suggest that CD107b may be involved in leucocyte adhesion.The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilisation procedure prior to staining. |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, IHC-Fr, IHC-P, IP, WB |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | M3/84 |
| 同位型 | IgG1 |
| 靶点名称 | CD107b / LAMP2 |
| 抗原物种 | Mouse |
| 抗原 | Glycoproteins purified from Mouse peritoneal macrophage membranes. |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD107b; LAMPB; CD107b; LGP110; Lysosome-associated membrane glycoprotein 2; LAMP-2; CD107 antigen-like family member B; Lysosome-associated membrane protein 2 |
应用说明
| 应用建议 |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P17047 Mouse Lysosome-associated membrane glycoprotein 2 |
|---|---|
| 基因名称 | Lamp2 |
| 全名 | lysosomal-associated membrane protein 2 |
| 背景介绍 | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008] |
| 生物功能 | Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. [UniProt] |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. |
检测图片 (1) Click the Picture to Zoom In
-
ARG22999 anti-CD107b / LAMP2 antibody [M3/84] WB image
Western blot: J774 cell lysate stained with ARG22999 anti-CD107b / LAMP2 antibody [M3/84] at 1:500, 1:1000 and 1:2000 dilution. Rat anti-Tubulin alpha is included as a loading control.
