ARG22183
anti-CD107b / LAMP2 antibody [H4B4]
anti-CD107b / LAMP2 antibody [H4B4] for Electron microscopy,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Purification,Western blot and Human,African green monkey,Rhesus Monkey
概述
| 产品描述 | Mouse Monoclonal antibody [H4B4] recognizes CD107b / LAMP2 |
|---|---|
| 反应物种 | Hu, AGMK, R. Mk |
| 应用 | EM, FACS, ICC/IF, IHC-Fr, IHC-P, IP, Puri, WB |
| 特异性 | Human/Rhesus/African Green Monkey CD107b |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | H4B4 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD107b / LAMP2 |
| 抗原物种 | Human |
| 抗原 | Human adherent peripheral blood cells |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD107b; LAMPB; CD107b; LGP110; Lysosome-associated membrane glycoprotein 2; LAMP-2; CD107 antigen-like family member B; Lysosome-associated membrane protein 2 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen retrieval: Heat tissue section in a microwave oven in 10mM Sodium citrate buffer (pH 6.0) twice for 10 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | BBS (pH 8.2) |
| 浓度 | 0.1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P13473 Human Lysosome-associated membrane glycoprotein 2 |
|---|---|
| 基因名称 | LAMP2 |
| 全名 | lysosomal-associated membrane protein 2 |
| 背景介绍 | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008] |
| 生物功能 | Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. [UniProt] |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. |
检测图片 (5) Click the Picture to Zoom In
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ARG22183 anti-CD107b / LAMP2 antibody [H4B4] ICC/IF image
Immunofluorescence: MYH1 siRNA transfected MCF7 cells stained with ARG22183 anti-CD107b / LAMP2 antibody [H4B4].
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ARG22183 anti-CD107b / LAMP2 antibody [H4B4] ICC/IF image
Immunofluorescence: RA differentiated SH-SY5Y neuroblastoma cells stained with ARG22183 anti-CD107b / LAMP2 antibody [H4B4] and anti-Aβ40, followed by AF546 and AF488 conjugated secondary antibodies and DAPI.
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ARG22183 anti-CD107b / LAMP2 antibody [H4B4] FACS image
Flow Cytometry: Jurkat cells was intracellularly stained with ARG22183 anti-CD107b / LAMP2 antibody [H4B4], followed by ARG21884 Goat anti-Mouse IgG1 antibody (FITC), pre-adsorbed.
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ARG22183 anti-CD107b / LAMP2 antibody [H4B4] IHC-Fr image
Immunohistochemistry: Frozen section of Mouse pancreas islets stained with ARG22183 anti-CD107b / LAMP2 antibody [H4B4] at 1:50 dilution.
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ARG22183 anti-CD107b / LAMP2 antibody [H4B4] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse pancreas islets stained with ARG22183 anti-CD107b / LAMP2 antibody [H4B4] at 1:100 dilution. Antigen retrieval: Heat tissue section in a microwave oven in 10mM Sodium citrate buffer (pH 6.0) twice for 10 min.
