ARG62700
anti-CD105 / Endoglin antibody [MEM-229]
anti-CD105 / Endoglin antibody [MEM-229] for Flow cytometry,ICC/IF,IHC-Frozen sections,Western blot and Human,Pig
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-229] recognizes CD105 / Endoglin |
|---|---|
| 反应物种 | Hu, Pig |
| 应用 | FACS, ICC/IF, IHC-Fr, WB |
| 特异性 | The clone MEM-229 recognizes CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-229 |
| 同位型 | IgG2a |
| 靶点名称 | CD105 / Endoglin |
| 抗原物种 | Human |
| 抗原 | Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA. |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD105; HHT1; Endoglin; ORW1; END |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Under non-reducing condition. IHC-Fr: Acetone fixation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||
| 阳性对照 | FACS: Kg1 human acute myelogenous leukemia cell line |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ENG |
| 全名 | endoglin |
| 背景介绍 | CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels. |
| 生物功能 | Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 71 kDa |
检测图片 (1) Click the Picture to Zoom In
-
ARG62700 anti-CD105 / Endoglin antibody [MEM-229] ICC/IF image
Immunofluorescence: Infarcted porcine heart stained with ARG62700 anti-CD105 / Endoglin antibody [MEM-229] (green).
Cell nuclei were stained with DAPI (blue).
克隆号文献
Disruption of endothelial adherens junctions by high glucose is mediated by protein kinase C-β-dependent vascular endothelial cadherin tyrosine phosphorylation.
A fat option for the pig: hepatocytic differentiated mesenchymal stem cells for translational research.
FACS / Pig
CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.
FACS / Human
Mitochondrial function and energy metabolism in umbilical cord blood- and bone marrow-derived mesenchymal stem cells.
FACS / Human
Tumor necrosis factor alpha promotes the expression of immunosuppressive proteins and enhances the cell growth in a human bone marrow-derived stem cell culture.
FACS / Human
Gadonanotubes as magnetic nanolabels for stem cell detection.
FACS / Pig
