ARG62699
anti-CD105 / Endoglin antibody [MEM-226]
anti-CD105 / Endoglin antibody [MEM-226] for CyTOF®-candidate,Flow cytometry,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-226] recognizes CD105 / Endoglin |
|---|---|
| 反应物种 | Hu |
| 应用 | CyTOF®-candidate, FACS, IP, WB |
| 特异性 | The clone MEM-226 reacts with CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-226 |
| 同位型 | IgG2a |
| 靶点名称 | CD105 / Endoglin |
| 抗原物种 | Human |
| 抗原 | Recombinant Vaccinia virus containing the human CD105 cDNA. |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD105; HHT1; Endoglin; ORW1; END |
应用说明
| 应用建议 |
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| 应用说明 | WB: Under non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ENG |
| 全名 | endoglin |
| 背景介绍 | CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels. |
| 生物功能 | Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 71 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG62699 anti-CD105 / Endoglin antibody [MEM-226] WB image
Western blot: 30 µg of HUVEC cell lysate stained with ARG62699 anti-CD105 / Endoglin antibody [MEM-226] at 1:500 dilution.
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ARG62699 anti-CD105 / Endoglin antibody [MEM-226] CyTOF image
CyTOF: hTERT cells were stained with ARG62699 anti-CD105 / Endoglin antibody [MEM-226] (143Nd). Singlet cells were gated for data analysis.
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anti-CD105 / Endoglin antibody [MEM-226]
Application：WB
Sample：HUVEC
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
克隆号文献
Conversion of human fibroblasts to angioblast-like progenitor cells.
An integrated transcriptional regulatory circuit that reinforces the breast cancer stem cell state.
Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.
FACS / Rat
Human ovarian carcinoma–associated mesenchymal stem cells regulate cancer stem cells and tumorigenesis via altered BMP production.
FACS / Human
Inducible formation of breast cancer stem cells and their dynamic equilibrium with non-stem cancer cells via IL6 secretion.
Puri / Human
The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitro.
FACS / Human
