ARG23944
anti-CD104 / Integrin beta 4 phospho (Tyr1494) antibody
anti-CD104 / Integrin beta 4 phospho (Tyr1494) antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD104 / Integrin beta 4 phospho (Tyr1494) |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD104 / Integrin beta 4 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated phosphospecific peptide around Tyr1494 of Human CD104 / Integrin beta 4. |
| 偶联标记 | Un-conjugated |
| 別名 | Integrin beta-4; GP150; CD antigen CD104; CD104 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using Integrin beta 4 phospho (Tyr1494) peptide (without carrier). |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGB4 |
| 全名 | integrin, beta 4 |
| 背景介绍 | Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Note=Colocalizes with DST at the leading edge of migrating keratinocytes. [UniProt] |
| 预测分子量 | 202 kDa |
| 翻译后修饰 | Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secundary association with tertaspanins. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG23944 anti-CD104 / Integrin beta 4 phospho (Tyr1494) antibody ICC/IF image
Immunofluorescence: A431 cells untreated (left) or treated with pervanadate (right). Cells were stained with ARG23944 anti-CD104 / Integrin beta 4 phospho (Tyr1494) antibody.
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ARG23944 anti-CD104 / Integrin beta 4 phospho (Tyr1494) antibody WB image
Western blot: A431 cells treated with serum starved overnight (left) and treated with pervanadate (1 mM) for 30 min (right). The blots were stained with ARG23944 anti-CD104 / Integrin beta 4 phospho (Tyr1494) antibody.
