ARG56551

anti-CCL2 / MCP1 antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

50 μg

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes CCL2 / MCP1
反应物种	Ms
应用	ELISA, IHC-P, Neut, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	CCL2 / MCP1
抗原物种	Mouse
抗原	E. coli derived recombinant Mouse CCL2 / MCP1.
偶联标记	Un-conjugated
別名	MCP1; AI323594; MCAF; Monocyte chemotactic protein 1; Sigje; Small-inducible cytokine A2; Platelet-derived growth factor-inducible protein JE; HC11; SMC-CF; JE; Scya2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

应用说明

应用建议

应用	推荐稀释比
ELISA	0.5 - 2.0 µg/ml
IHC-P	0.5 - 2.0 µg/ml
Neut	4.0 - 6.0 µg/ml (to yield [ND50] of the biological activity of MCP-1 (100 ng/ml))
WB	0.1 - 0.2 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.2)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 20296 Mouse CCL2 Swiss-port # P10148 Mouse C-C motif chemokine 2
基因名称	Ccl2
全名	chemokine (C-C motif) ligand 2
背景介绍	This gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. [provided by RefSeq, Sep 2015]
生物功能	Chemotactic factor that attracts monocytes, but not neutrophils. [UniProt]
产品亮点	Related products: MCP1 antibodies; MCP1 ELISA Kits; MCP1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines
预测分子量	11 kDa
翻译后修饰	Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

检测图片 (4) Click the Picture to Zoom In

ARG56551 anti-CCL2 / MCP1 antibody IHC image

Immunohistochemistry: This antibody stained colchicine injected Mouse brain (including caudate putamen) tissue. The primary antibody ARG56551 anti-CCL2 / MCP1 antibody was incubated at 1.0 µg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification reagent.
ARG56551 anti-CCL2 / MCP1 antibody WB image

Western blot: 250 - 0.24 ng (left to right) of recombinant Mouse MCP-1 stained with ARG56551 anti-CCL2 / MCP1 antibody. (Under non-reducing conditions)
ARG56551 anti-CCL2 / MCP1 antibody standard curve image

ARG56551 anti-CCL2 / MCP1 antibody results of a typical standard run with optical density reading at 405 - 650 nm.
ARG56551 anti-CCL2 / MCP1 antibody WB image

Western blot: 250 - 0.24 ng (left to right) of recombinant Mouse MCP-1 stained with ARG56551 anti-CCL2 / MCP1 antibody. (Under reducing conditions)